Journal
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
Volume 45, Issue 3, Pages 451-458Publisher
WILEY
DOI: 10.1016/j.femsim.2005.05.019
Keywords
infected bats; Histoplasma capsulatum; RAPD-patterns; DNA sequences
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Fourteen Histoplasma capsulatum isolates recovered from infected bats captured in Mexican caves and two human H. capsulatum reference strains were analyzed using random amplification of polymorphic DNA PCR-based and partial DNA sequences of four genes. Cluster analysis of random amplification of polymorphic DNA-patterns revealed differences for two H. capsulatum isolates of one migratory bat Tadarida brasiliensis. Three groups were identified by distance and maximum-parsimony analyses of arf, H-anti, ale, and tub1 H. capsulatum genes. Group I included most isolates from infected bats and one clinical strain from central Mexico; group II included the two isolates from T brasiliensis; the human G-217B reference strain from USA formed an independent group III. Isolates from group II showed diversity in relation to groups I and III , suggesting a different H. capsulatum population. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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