Journal
SCIENCE
Volume 309, Issue 5740, Pages 1584-1587Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1113645
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Twin-ribozyme introns are formed by two ribozymes belonging to the group I family and occur in some ribosomal RNA transcripts. The group l-like ribozyme, GIR1, liberates the S' end of a homing endonuclease messenger RNA in the slime mold Didymium iridis. We demonstrate that this cleavage occurs by a transesterification reaction with the joining of the first and the third nucleotide of the messenger by a 2',5'-phosphodiester linkage, Thus, a group l-like ribozyme catalyzes an RNA branching reaction similar to the first step of splicing in group II introns and spliceosomal introns. The resulting short lariat, by forming a protective 5' cap, might have been useful in a primitive RNA world.
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