Journal
VETERINARY PARASITOLOGY
Volume 132, Issue 1-2, Pages 43-49Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2005.05.018
Keywords
Trichinella; 2D-electrophoresis; mass spectrometry; antigens
Categories
Ask authors/readers for more resources
A proteomic approach was utilized for fine antigenic characterization of the closely related Trichinella genotypes Trichinella britovi T3 and Trichinella T8. Crude extract of muscle larvae L1 (LCE) from both isolates were analyzed by 213-PAGE. Over 500 protein spots were reproducibly separated in both genotypes. These separated proteins were identified in Western blot with IgG1 and IgG3 from homologous and heterologous hyperimmune sera raised in BALB/c mice. A group of 20 and 15 spots migrating at 50-60 kDa and pH 5.5-6.5 in T britovi and Trichinella T8 maps, respectively, reacted with the IgGI from heterologous sera whereas a group of minor spots of similar migration patterns did not. Low cross-reactivity occurred for IgG3. MALDI-TOF and MALDI-TOF/TOF analysis of these antigens identified a predicted enolase, the protein P49 and a predicted actin among the cross-reactive proteins and two hypothetical actins among the non cross-reactive proteins. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available