Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 823, Issue 2, Pages 143-151Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2005.06.024
Keywords
flavonoids; phenolic acids; polyphenols; HPLC; biomarker; diet
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We developed a sensitive method for determination of 23 flavonoids and phenolic acids, which represent phenolic acids and five subclasses of flavonoids. Plasma samples were extracted with selective solid-phase-extraction columns and separated by RP-high-performance liquid chromatography (HPLC). For detection an electrochemical detector was used. Identification and test of purity were carried out via retention times and spectra analyses. Limits of detection varied from 1.45 to 22.27 nmol/l. Recovery varied from 81% to 106%. Reproducibility for all analytes was below 10% (coefficient of variation, CV (%)) and ranged between 3.1% and 9.8%. This method can be applied to samples from interventional studies as well as observational studies. (c) 2005 Elsevier B.V. All rights reserved.
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