Journal
CHEMISTRY OF MATERIALS
Volume 17, Issue 18, Pages 4636-4641Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cm050935k
Keywords
-
Ask authors/readers for more resources
Gold nanorods have been stabilized, conjugated to antibodies, and characterized for biological applications. The stabilizing surfactant bilayer which surrounds gold nanorods was replaced by thiol terminated methoxypoly (ethylene glycol) so that the nanorods are stable in buffer solutions free of surfactant. Nanorod bioconjugation was accomplished with a heterobifunctional cross-linker, with antibody activity confirmed by a strip plate assay. Nanorod bioconjugates were characterized by independent measurements of the nanorod and antibody concentrations. The nanorod molar extinction coefficient was measured from dense yet well separated nanorod films to be 4.4 +/- 0.5 x 10(9) M cm(-1), thus allowing quantitative determination of nanorod concentrations. However, a colorimetric protein assay overestimated the antibody concentration and is therefore likely perturbed by the presence of the nanorods electromagnetically and/or chemically.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available