Journal
EMBO JOURNAL
Volume 24, Issue 17, Pages 3057-3069Publisher
WILEY
DOI: 10.1038/sj.emboj.7600765
Keywords
intron bridging; pre-mRNA splicing; U11/U12; snRNP; U12-type spliceosome
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U11 and U12 interact cooperatively with the 50 splice site and branch site of pre-mRNA as a stable preformed di-snRNP complex, thereby bridging the 5' and 3' ends of the intron within the U12-dependent prespliceosome. To identify proteins contributing to di-snRNP formation and intron bridging, we investigated protein - protein and protein - RNA interactions between components of the U11/ U12 snRNP. We demonstrate that the U11/ U12-65K protein possesses dual binding activity, interacting directly with U12 snRNA via its C-terminal RRM and the U11-associated 59K protein via its N-terminal half. We provide evidence that, in contrast to the previously published U12 snRNA secondary structure model, the 30 half of U12 forms an extended stem-loop with a highly conserved seven-nucleotide loop and that the latter serves as the 65K binding site. Addition of an oligonucleotide comprising the 65K binding site to an in vitro splicing reaction inhibited U12-dependent, but not U2-dependent, pre-mRNA splicing. Taken together, these data suggest that U11/ U12-65K and U11-59K contribute to di-snRNP formation and intron bridging in the minor prespliceosome.
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