Journal
DEVELOPMENTAL BIOLOGY
Volume 285, Issue 2, Pages 570-583Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2005.06.033
Keywords
proneural; bHLH transcription factor; neuronal differentiation; target genes
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Funding
- NEI NIH HHS [R01 EY012274, EY12274, R01 EY012274-10] Funding Source: Medline
- NICHD NIH HHS [1T32HD07491] Funding Source: Medline
- PHS HHS [07464 28] Funding Source: Medline
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Proneural basic helix-loop-helix (bHLH) transcription factors are critical positive regulators of neuronal differentiation in a variety of species and are required for proper differentiation of various subtypes of neurons. Although bHLH factors demonstrate some unique functions during neural development, they share the ability to regulate neuronal differentiation, potentially by targeting overlapping sets of genes. To assess this, we performed a screen in ectoderm animal cap tissue to identify direct transcriptional targets shared by two Xenopus ato-related bHLH factors, Xath5 and X-NeuroD. Candidate target genes identified in this screen include several transcriptional regulators (Xebf2, Xebf3, XETOR and NKL), an RNA binding protein (e1rC), a cell cycle component (Xgadd45 gamma) and several novel genes. Overexpression of either Xath5 or XNeuroD induced ectopic in vivo expression of these candidate target genes. Conversely, blocking ato-related bHLH activity prevented endogenous nervous system expression of these genes. Therefore, we have identified a set of genes that can be regulated by multiple ato-related bHLH factors and may function as critical effectors of proneural bHLH-mediated differentiation. (c) 2005 Elsevier Inc. All rights reserved.
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