Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 102, Issue 38, Pages 13383-13385Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0506290102
Keywords
DNA-DNA hybridization; monolayer-protected gold clusters; nanostructure
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Glutathione monolayer-protected gold clusters were reacted by place exchange with 19 or 20-residue thiolated oligonucleoticles. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleoticles by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.
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