Journal
CYTOKINE
Volume 31, Issue 6, Pages 429-438Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2005.06.015
Keywords
cytokine; inflammation; RT-PCR; wound healing
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Inflammatory mediators have been shown to play a major role in the complex series of co-ordinated events that occur in wound healing responses following injury. However, to date most of the studies carried out have addressed the expression, interactions and role of only one or two cytokines that are thought to be involved in wound repair. This study has evaluated, in murine skin samples taken at 0, 3, 12, 18, 24, 48, 72, 120 and 168 h post-wounding, the expression of a wide range of cytokines with potential for a role in wound repair. Various techniques (reverse transcription polymerase chain reaction (RT-PCR), bioassays and ELISA) were used to evaluate cytokine expression in these samples at both the mRNA and protein expressions level. Semi-quantitative analysis using RTPCR revealed that IL-1 beta, IP10, bFGF, and TGF beta 3 up-regulated in wounded samples, compared to non-injured control samples. Expression of mRNA for other cytokines and inflammatory mediators, IL-l alpha, IL-6, TGF beta I, TNF alpha, MIP-1 alpha, MIP-2, JE, KC, PDGF alpha and PDGF beta, were found to be down-regulated in injured adult murine samples compared to normal control samples. Interestingly we failed to find evidence of mRNA expression for the cytokines IL-2, IL-4, IL-12, GM-CSF, IFN gamma and RANTES, in both non-injured and injured samples. These observations were also generally supported by the results obtained using bioassays for IL-1 and IL-6 and ELISA for IL-la, IL-1 beta, IL-6, TNFa, and IFN gamma. (c) 2005 Elsevier Ltd. All rights reserved.
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