Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 280, Issue 38, Pages 32578-32585Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M503510200
Keywords
-
Categories
Funding
- NCI NIH HHS [P30 CA 1314] Funding Source: Medline
- NIAID NIH HHS [AI054496, AI39033] Funding Source: Medline
Ask authors/readers for more resources
Human innate immunity to non- pathogenic species of African trypanosomes is provided by human high density lipoprotein ( HDL) particles. Here we show that native human HDLs containing hapto- globin- related protein ( Hpr), apolipoprotein L- I ( apoL- I) and apolipoprotein A- I ( apoA- I) are the principle antimicrobial molecules providing protection from trypanosome infection. Other HDL subclasses containing either apoA- I and apoL- I or apoA- I and Hpr have reduced trypanolytic activity, whereas HDL subclasses lacking apoL- I and Hpr are non- toxic to trypanosomes. Highly purified, lipid- free Hpr and apoL- I were both toxic to Trypanosoma brucei brucei but with specific activities at least 500- fold less than those of native HDLs, suggesting that association of these apolipoproteins within the HDL particle was necessary for optimal cytotoxicity. These studies show that HDLs can serve as platforms for the assembly of multiple synergistic proteins and that these assemblies may play a critical role in the evolution of primate- specific innate immunity to trypanosome infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available