Journal
SCIENCE
Volume 309, Issue 5743, Pages 2064-2067Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1114758
Keywords
-
Categories
Ask authors/readers for more resources
Muscle satellite cells contribute to muscle regeneration. We have used a Pax3(GFP/+) mouse line to directly isolate (Pax3)(green fluorescent protein)-expressing muscle satellite cells, by flow cytometry from adult skeletal muscles, as, a homogeneous population of small, nongranular, Pax7+, CD34+, CD45-, Sca1-cells. The flow cytometry parameters thus established enabled us to isolate satellite cells from wild-type muscles. Such cells, grafted into muscles of mdx nu/nu mice, contributed both to fiber repair and to the muscle satellite cell compartment. Expansion of these cells in culture before engraftment reduced their regenerative capacity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available