Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 824, Issue 1-2, Pages 116-122Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2005.07.006
Keywords
aminoacetone; SSAO; methylglyoxal; HPLC
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Increase in methylglyoxal is thought to be involved in different pathological conditions. Deamination of aminoacetone by semicarbazidesensitive amine oxidase (SSAO) leads to production of methylglyoxal. We have synthesized aminoacetone and developed a novel HPLC procedure for its quantitative determination. The urinary excretion of aminoacetone is approximately 20-30 mu g/mouse/day, and the concentration is about 0.5 mu g/g in mouse liver and small intestine. SSAO inhibitor increases aminoacetone levels in both tissues and urines. Results confirm that aminoacetone is an endogenous substrate for SSAO. However, data also indicate that deamination is not the only catabolic pathway for aminoacetone. (c) 2005 Elsevier B.V. All rights reserved.
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