Journal
FEBS LETTERS
Volume 579, Issue 23, Pages 5217-5222Publisher
WILEY
DOI: 10.1016/j.febslet.2005.07.099
Keywords
cell-penetrating peptides; internalization; degradation; Saccharomyces cerevisiae; Candida albicans
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The uptake of different cell-penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans, was studied using fluorescence HPLC-analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF)(3)K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studies. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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