Mzabimyces algeriensis gen. nov., sp nov., a halophilic filamentous actinobacterium isolated from a Saharan soil, and proposal of Mzabimycetaceae fam. nov.

Three halophilic mycelium-forming actinobacteria, strains H195(T), H150 and H151, were isolated from a Saharan soil sample collected from Beni-isguen in the Mzab region (Ghardaia, South of Algeria) and subjected to a polyphasic taxonomic characterisation. These strains were observed to show an aerial mycelium differentiated into coccoid spore chains and fragmented substrate mycelium. Comparative analysis of the 16S rRNA gene sequences revealed that the highest sequence similarities were to Saccharopolyspora qijiaojingensis YIM 91168(T) (92.02 % to H195(T)). Phylogenetic analyses showed that the strains H195(T), H150 and H151 represent a distinct phylogenetic lineage. The cell-wall hydrolysate was found to contain meso-diaminopimelic acid, and the diagnostic whole-cell sugars were identified as arabinose and galactose. The major cellular fatty acids were identified as iso-C-15:0, iso-C-16:0, iso-C-17:0 and anteiso-C-17:0. The diagnostic phospholipid detected was phosphatidylcholine and MK-9 (H-4) was found to be the predominant menaquinone. The genomic DNA G+C content of strain H195(T) was 68.2 mol%. On the basis of its phenotypic features and phylogenetic position, we propose that strain H195(T) represents a novel genus and species, Mzabimyces algeriensis gen. nov., sp. nov., within a new family, Mzabimycetaceae fam. nov. The type strain of M. algeriensis is strain H195(T) (= DSM 46680(T) = MTCC 12101(T)).