Journal
NATURE CHEMICAL BIOLOGY
Volume 1, Issue 5, Pages 263-264Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nchembio732
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Funding
- NHLBI NIH HHS [5T32HL07208-26] Funding Source: Medline
- NIA NIH HHS [1K01AG023562-01, K01 AG023562] Funding Source: Medline
- NIGMS NIH HHS [R21 GM075946] Funding Source: Medline
- NINDS NIH HHS [1R03NS050806-01, R03 NS050806] Funding Source: Medline
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To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
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