Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 103, Issue 6, Pages 1321-1327Publisher
SPRINGER
DOI: 10.1007/s10482-013-9913-5
Keywords
Tenacibaculum halocynthiae sp nov.; Novel species; Polyphasic taxonomy; Sea squirt (Halocynthia roretzi)
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Funding
- National Fisheries Research and Development Institute (NFRDI)
- Program for Collection, Management and Utilization of Biological Resources
- BK21 program from the Ministry of Education, Science & Technology (MEST) of the Republic of Korea
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A Gram-negative, non-spore-forming, aerobic, non-flagellated, non-gliding and rod-shaped bacterial strain, designated P-R2A1-2(T), was isolated from sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. It grew optimally at 25-28 A degrees C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that the strain fell within the clade comprising Tenacibaculum species. Strain P-R2A1-2(T) exhibited the highest 16S rRNA gene sequence similarity values of 97.6, 97.2 and 97.0 % to Tenacibaculum aestuarii SMK-4(T), T. lutimaris TF-26(T) and T. aiptasiae a4(T), respectively, and of 94.5-96.8 % to the type strains of the other Tenacibaculum species. Strain P-R2A1-2(T) contained MK-6 as the predominant menaquinone and C-16:1 omega 7c and/or iso-C-15:0 2-OH, iso-C-15:0 3-OH and iso-C-15:0 as the major fatty acids. The DNA G + C content of strain P-R2A1-2(T) was 30.7 mol % and its DNA-DNA relatedness values with the type strains of T. aestuarii, T. lutimaris and T. aiptasiae were 17 +/- A 4.2, 21 +/- A 6.1 and 16 +/- A 5.2 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that the novel strain is separate from other Tenacibaculum species. On the basis of the data presented, strain P-R2A1-2(T) is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum halocynthiae sp. nov. is proposed. The type strain is P-R2A1-2(T) (=KCTC 32262(T) = CCUG 63681(T)).
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