Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 11, Issue 10, Pages 834-837Publisher
ELSEVIER SCI LTD
DOI: 10.1111/j.1469-0691.2005.01236.x
Keywords
methicillin-resistant Staphylococcus aureus; MRSA; orfX; PCR; typing; SCCmec
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The chromosomal location of the SCCmec elements containing mecA allows the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains by PCR amplification of a sequence covering the right junction of the SCCmec elements and the adjacent chromosomal region encoding the species-specific ORFX. MRSA strains can be identified specifically using one forward primer, with only one or two mismatches, targeting the SCCmec elements of different types, and one reverse primer targeting the orfX region.
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