Journal
CONTRACEPTION
Volume 72, Issue 4, Pages 298-302Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.contraception.2005.04.011
Keywords
c-Flip; testis; spermatocytes; spermatogonia; caspases
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The aim of the present study was to investigate the expression and role of c-Flip long isoform (c-Flip(L)), a known anti-apoptotic protein. No data are currently available on c-Flip(L) in male gonad before puberty; therefore, this study was carried out in prepuberal mouse testis. We investigated pachytene spermatocytes and spermatogonia by immunostaining of testis sections and found a strong and specific expression of c-Flip(L) in pachytene spermatocytes, while spermatogonia expressed very low levels of c-Flip(L). This finding inversely correlated with the caspases activity, which was higher in spermatogonia as compared to pachytene spermatocytes. Other experiments carried out in an organ-culture model revealed that Fas-induced apoptosis was higher in spermatogonia as compared to pachytene spermatocytes. These data suggest that c-Flip(L) may play a role as an anti-apoptotic molecule in the prepuberal mouse testis and open new perspectives in the comprehension of the mechanisms controlling germ cells apoptosis. (c) 2005 Elsevier Inc. All rights reserved.
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