Journal
EXPERIMENTAL PARASITOLOGY
Volume 111, Issue 2, Pages 126-129Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.exppara.2005.05.008
Keywords
Trypanosoma vivax; purification; immunosuppres-ovine; trypanosomosis
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Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and inummosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts. (c) 2005 Elsevier Inc. All rights reserved.
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