Journal
NANO LETTERS
Volume 5, Issue 10, Pages 1883-1888Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl0510816
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Funding
- NCRR NIH HHS [P41 RR005969, P41 RR005969-11, P41 RR 05969] Funding Source: Medline
- NHGRI NIH HHS [R01 HG 003713-01] Funding Source: Medline
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The mechanical properties of DNA over segments comparable to the size of a protein-binding site (3-10 nm) are examined using an electric-field-induced translocation of single molecules through a nanometer diameter pore. DNA, immersed in an electrolyte, is forced through synthetic pores ranging from 0.5 to 1.5 nm in radius in a 10 nm thick Si3N4 membrane using an electric field. To account for the stretching and bending, we use molecular dynamics to simulate the translocation. We have found a threshold for translocation that depends on both the dimensions of the pore and the applied transmembrane bias. The voltage threshold coincides with the stretching transition that occurs in double-stranded DNA near 60 pN.
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