Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 43, Issue 10, Pages 5332-5337Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.43.10.5332-5337.2005
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By reexamining 10 previously published universal PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.
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