Journal
ANTIVIRAL RESEARCH
Volume 96, Issue 3, Pages 324-332Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2012.10.002
Keywords
Attenuated vaccinia virus vector; Tian Tan strain; TE3L; Virulence; Immunogenicity
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Funding
- Genetically Modified Organisms Breeding Major Projects [2009ZX08006-002B]
- Key Technologies R&D Programme of Jilin Province [10ZDGG007]
- National Natural Science Foundation of China [81101140]
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An attenuated vaccinia virus (VACV), TE3L(-)VTT, was evaluated for virulence and safety to determine its potential use as a vaccine or as a recombinant virus vector to express foreign genes. The virulence of TE3L(-)VTT was compared with that of the wild-type VTT both in vivo and in vitro. The humoral and cellular immune responses were detected in a mouse model to test the vaccine efficacy of the TE3L mutant. The results suggested that deletion of the TE3L gene decreased the virulence and neurovirulence significantly in mice and rabbit models, yet retained the immunogenicity. Thus, the deletion of TE3L improved the safety of the VTT vector: this approach may yield a valuable resource for studies of recombinant VACV-vectored vaccines. (C) 2012 Elsevier B.V. All rights reserved.
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