Journal
FISHERIES SCIENCE
Volume 71, Issue 5, Pages 987-991Publisher
SPRINGER JAPAN KK
DOI: 10.1111/j.1444-2906.2005.01055.x
Keywords
Alexandrium tamarense; cyst; dinoflagellate; paralytic shellfish poisoning (PSP); real-time PCR
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The cysts of toxic dinoflagellate Alexandrium tamarense are the seed population for the bloom responsible for paralytic shellfish poisoning (PSP). However, it is impossible to identify the Alexandrium spp. cyst on the basis of morphological features. In this study, we prepared A. tamarense cysts by sexual conjugation in laboratory conditions and developed an efficient DNA extraction method for polymerase chain reaction (PCR) assay. Using the A. tamarense cysts, we established the identification and quantification method showing the species specificity and the high sensistivity for A. tamarense cysts using real-time PCR. This assay was also able to detect and quantify the A. tamarense cysts accurately when mixed with excess cysts of A. catenella (Whedon and Kofoid) Balech prepared by conjugation experiment.
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