Journal
ANTIVIRAL RESEARCH
Volume 89, Issue 1, Pages 98-108Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.antiviral.2010.11.012
Keywords
Herpes simplex virus type 1; Pentagalloylglucose; Two-dimensional gel electrophoresis; Actin cytoskeleton; Cofilin1
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Funding
- National Science Foundation of China [U0632010]
- State Key Laboratory of Phytochemistry and Plant Resources in West China
- Kunming Institute of Botany
- Chinese Academy of Sciences [P2008-KF07, P2008-ZZ08]
- Ministry of Education (MOE)
- Fundamental Research Funds for the Central Universities
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To investigate the anti-herpesvirus mechanism of pentagalloylglucose (PGG), we compared the proteomic changes between herpes simplex virus type 1 (HSV-1) infected MRC-5 cells with or without PGG-treatment, and between non-infected MRC-5 cells with or without PGG-treatment by 2-DE and MS-based analysis. Differentially expressed cellular proteins were mainly involved with actin cytoskeleton regulation. Significantly, PGG can down-regulate cofilin1, a key regulator of actin cytoskeleton dynamics. PGG can inhibit HSV-1-induced rearrangements of actin cytoskeleton which is important for infectivity. Furthermore, cofilin1 knockdown by siRNA also inhibited the HSV-1-induced actin-skeleton rearrangements. Both PGG-treatment and cofilin1 knockdown can reduce HSV-1 DNA, mRNA, protein synthesis and virus yields. Altogether, the results suggested that down-regulating cofilin1 plays a role in PGG inhibiting HSV-1 infection. PGG may be a promising anti-herpesvirus agent for drug development. (C) 2010 Elsevier B.V. All rights reserved.
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