Journal
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Volume 53, Issue 2, Pages 121-124Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2005.05.011
Keywords
MIC; NRA; ABA; agar proportion method; Mycobacterium tuberculosis
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A rapid colorimetric, microplate-based nitrate reductase assay (NRA) method for antibiotic susceptibility profile of clinical isolates of Mycobacterium tuberculosis was compared with Alamar Blue assay (ABA). The results obtained by both the methods were also compared with conventional agar proportion method. The overall agreement between the results obtained by NRA and ABA was 99% and 98%, respectively, when compared with agar proportion method. Reproducible results for all the isolates were obtained within 8 days by NRA as well as ABA. The specificity of NRA method for M. tuberculosis makes it more suitable method for MIC determination. (c) 2005 Elsevier Inc. All rights reserved.
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