Journal
RNA
Volume 11, Issue 10, Pages 1555-1562Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.2121705
Keywords
trans-acting ribozyme; in vitro compartmentalization; in vitro selection
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Funding
- NIBIB NIH HHS [8R01 EB002043, R01 EB002043] Funding Source: Medline
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We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis.
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