Journal
ANTIVIRAL RESEARCH
Volume 80, Issue 2, Pages 158-167Publisher
ELSEVIER
DOI: 10.1016/j.antiviral.2008.06.006
Keywords
Morbillivirus; RNA interference; Real-time PCR; Measles; Rinderpest; Peste des petits ruminants
Categories
Funding
- EU Pan-African program for the Control of Epizootics [REG/5005/005]
- International Atomic Energy Agency [RAF/05/053]
- EPIZONE Network of Excellence [016236-FOOD]
- French Ministry of Foreign Affairs, French Embassy, Cote d'Ivoire [DSRV/CIV/481/017]
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The morbillivirus genus includes important pathogens such as measles virus (MV), peste des petits ruminants virus (PPRV), and rinderpest virus (RPV) and forms a group of antigenically related viruses, The viral nucleoprotein (N) is a well-conserved protein among the genus and plays a central role in the replication of the virus. Using a comprehensive approach for siRNA screening of the conserved sequences of the N gene, including sequence analysis and functional in vitro tests, we have identified a region for the design of siRNA effective for the control of PPRV, RPV, and MV replication. Silencing of the N mRNA efficiently shuts down the production of N transcripts, the expression of N protein, and the indirect inhibition of matrix protein, resulting in the inhibition of PPRV progeny by 10,000-fold. These results suggest that siRNA against this region should be further explored as a therapeutic strategy for morbillivirus infections. (C) 2008 Elsevier B.V. All rights reserved.
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