Journal
JOURNAL OF SEPARATION SCIENCE
Volume 28, Issue 15, Pages 1956-1968Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200500258
Keywords
glycomics; glycoproteomics; LC/MALDI/TOF/TOF MS; LC/MS; lectin; oligosaccharides; online permethylation; review; tandem mass spectrometry
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Funding
- NIGMS NIH HHS [GM24349, R01 GM024349] Funding Source: Medline
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High-sensitivity glycoprotein analyses are of particular interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of new hyphenated methodologies in high-sensitivity glycoprotein analysis is highlighted in this thematic review. These methodologies include, in particular, capillary LC/MALDI/TOF/TOF MS in conjunction with online permethylation platform, and silica-based lectin microcolumns interfaced to MS. The potential of these methodologies in glycomic and glycoproteomic analysis is demonstrated for model glycoproteins as well as total glycomes and glycoproteomes derived from biological samples. Additionally, the applications of CE-MS, CEC, and nanoLC with graphitized carbon in the areas of glycomics and glycoproteomics are described.
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