Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 25, Issue 10, Pages 1312-1324Publisher
SAGE PUBLICATIONS INC
DOI: 10.1038/sj.jcbfm.9600124
Keywords
cDNA array; cerebral ischemia; chemokine; gene; inflammation; superoxide
Categories
Funding
- NINDS NIH HHS [R01 NS25372, R01 NS36147, R01 NS38653, P50 NS14543] Funding Source: Medline
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Proinflammatory cytokines and chemokines are quickly upregulated in response to ischemia/reperfusion (I/R) injury; however, the relationship between I/R-induced oxidative stress and cytokine/chemokine expression has not been elucidated. We investigated the temporal profile of cytokine and chemokine gene expression in transient focal cerebral ischemia using complementary DNA array technology. Among 96 genes studied, 10, 4, 11, and 5 genes were increased at 6, 12, 24, and 72 h of reperfusion, respectively, whereas, 4, 11, 8, and 21 genes, respectively, were decreased. To clarify the relationship between chemokines and oxidative stress, we compared the gene and protein expression of monocyte chemoattractant protein 1 (MCP-1) and macrophage inflammatory protein-1 alpha (MIP-1 alpha) in wild-type (WT) mice and copper/zinc-superoxide dismutase (SOD1) transgenic (Tg) mice. Monocyte chemoattractant protein-1 alpha and MIP-1 alpha mRNA were significantly upregulated at 6 to 12 h of reperfusion. In the SOD1 Tg mice, however, MCP-1 and MIP-1 alpha mRNA expression was significantly decreased 12h postinsult. In the WT mice, MCP-1 and MIP-1 alpha, protein expression peaked 24 h after onset of reperfusion determined by immunohistochemistry. In the SOD1 Tg mice, MCP-1 and MIP-1 alpha immunopositive cells were reduced, as were concentrations of these proteins (measured by enzyme-linked immunosorbent assay) at 24 h of reperfusion. Our results suggest that MCP-1 and MIP-1 alpha expression is influenced by I/R-induced oxidative stress after transient focal stroke.
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