Journal
ELECTROPHORESIS
Volume 26, Issue 19, Pages 3641-3649Publisher
WILEY
DOI: 10.1002/elps.200500246
Keywords
mass spectrometry; microfluidics; miniaturization; nanoliquid chromatography; oligosaccharides
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The nanoLC separations of oligosaccharides using microchip-based columns are described. Mixtures of alditols from mucins and human milk are separated on graphitized carbon. The nanoLC-MS device showed high mass accuracy for the oligosaccharides ranging between 1 and 6 ppm on routine analyses. The high mass accuracy readily allowed identification of oligosaccharicle peaks and the determination of their compositions. High retention time reproducibility was exhibited by the microchip LC. Little variation was observed for standard sample either alone or in a complex heterogeneous mixture. The nanoLC-MS exhibits excellent capabilities in profiling mixtures of oligosaccharides.
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