4.5 Article

An improved method for marine cyanobacterial DNA isolation

Journal

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 21, Issue 6-7, Pages 877-881

Publisher

SPRINGER
DOI: 10.1007/s11274-004-6187-0

Keywords

DNA isolation; improved method; marine cyanobacteria; modification; RAPD-PCR

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The method of Bolch, Blackburn, Jones, Orr & Grewe [Phycologia, 36, 6 11, 1997] developed for isolation of DNA from freshwater cyanobacteria was suitably modified to yield a simple, efficient and reproducible protocol for the isolation of DNA from different morphological types of marine cyanobacteria. This method resulted in a high yield of quality DNA suitable for polymerase chain reaction (PCR) amplifications.

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