Journal
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
Volume 127, Issue 5, Pages 751-757Publisher
ASME
DOI: 10.1115/1.1993661
Keywords
cell viability; cell adhesion; HSP70 expression; thermal stress; thermal injury
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Thermal preconditioning protocols for cardiac cells were identified which produce elevated HSP70 levels while maintaining, high cell viability. Bovine aortic endothelial cells were heated with a water bath at temperatures ranging from 44 to 50 degrees C for periods of 1-30 min. Thermal stimulation protocols were determined which induce HSP70 expression levels ranging from 2.3 to 3.6 times the control while maintaining cell viabilities greater than 90%. An Arrhenius injury model fit to the cell damage data yielded values of A = 1.4 x 10(66) s(-1) and E-a = 4.1 x 10(5) J/mol. Knowledge of the injury parameters and HSP70 kinetics will enhance dosimetry guideline development for thermal stimulation heat shock proteins expression in cardiac tissue.
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