Journal
PLANTA
Volume 222, Issue 2, Pages 301-306Publisher
SPRINGER
DOI: 10.1007/s00425-005-1524-6
Keywords
Arabidopsis; 5-azacytidine; ddm1 mutant; DNA methylation pattern; plant development
Categories
Ask authors/readers for more resources
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available