Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 33, Issue 2, Pages 95-104Publisher
SPRINGER
DOI: 10.1007/s10858-005-2063-y
Keywords
inclusion bodies; isotope enrichment; lysozyme; non-native proteins; resonance assignment; tryptophan side chains
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A new protocol is described for the isotope (N-15 and C-13,N-15) enrichment of hen egg white lysozyme. Hen egg white lysozyme and an all-Ala-mutant of this protein have been expressed in E. coli. They formed inclusion bodies from which mg quantities of the proteins were purified and prepared for NMR spectroscopic investigations. (HC)-H-1-C-13 and N-15 main chain resonances of disulfide reduced and S-methylated lysozyme were assigned and its residual structure in water pH 2 was characterized by chemical shift perturbation analysis. A new NMR experiment has been developed to assign tryptophan side chain indole resonances by correlation of side chain and backbone NH resonances with the C-gamma supercript stop resonances of these residues. Assignment of tryptophan side chains enables further residue specific investigations on structural and dynamical properties, which are of significant interest for the understanding of non-natives states of lysozyme stabilized by hydrophobic interactions between clusters of tryptophan residues.
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