Journal
BIOMATERIALS
Volume 26, Issue 28, Pages 5624-5631Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2005.02.014
Keywords
biomimetic material; nanotopography; ECM; cell culture
Funding
- NINDS NIH HHS [R01 NS40394] Funding Source: Medline
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The purpose of this study was to design a synthetic nanofibrillar matrix that more accurately models the porosity and fibrillar geometry of cell attachment surfaces in tissues. The synthetic nanofibrillar matrices are composed of nanofibers prepared by electrospinning a polymer Solution of polyamide onto glass coverslips. Scanning electron and atomic force microscopy showed that the nanofibers were organized into fibrillar networks reminiscent of the architecture of basement membrane, a structurally compact form of the extracellular matrix (ECM). N1H 3T3 fibroblasts and normal rat kidney (NRK) cells. when grown on nanofibers in the presence of serum, displayed the morphology and characteristics of their Counterparts in vivo. Breast epithelial cells underwent morphogenesis to form multicellular spheroids containing lumens. Hence the synthetic nanolibrillar matrix described herein provides a physically and chemically stable three-dimensional surface for ex vivo growth of cells. Nanofiber-based synthetic matrices could have considerable value for applications in tissue engineering. cell-based therapies, and studies of cell/tissue function and pathology. (c) 2005 Elsevier Ltd. All rights reserved.
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