Journal
NEUROCHEMICAL RESEARCH
Volume 30, Issue 10, Pages 1269-1281Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-005-8798-8
Keywords
ammonia detoxification; anaplerosis; glutamine; hyperammonemia; multinuclear NMR spectroscopy; neuronal metabolism
Categories
Ask authors/readers for more resources
Multinuclear NMR spectroscopy is used to investigate the effect of glutamine on neuronal glucose metabolism. Primary neurons were incubated with [1-C-13]glucose in the absence or presence of glutamine (2 mM) and/or NH4Cl (5 mM). After ammonia-treatment, the concentrations of high-energy phosphates decreased up to 84% of control, which was aggravated in glutamine-containing medium (up to 42% of control). These effects could not be attributed to changes in mitochondrial glucose oxidation. Withdrawal of glutamine decreased amino acid concentrations, e.g. of glutamate to 53%, but also considerably lessened the C-13 enrichment in [4-C-13]glutamate to 8.3% of control, and decreased the C-13-enrichment in acetyl-CoA entering the Krebs cycle (P < 0.001). Thus, although glutamine is potent in replenishing neuronal glutamate stores, glutamate formation is mainly attributed to its de novo synthesis from glucose. Furthermore, mitochondrial glucose metabolism strongly depends on the supply of carbons from glutamine, indicating that exogenous glutamine is a well-suited substrate to replenish neuronal Krebs cycle intermediates.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available