Journal
CHROMATOGRAPHIA
Volume 62, Issue 7-8, Pages 435-439Publisher
VIEWEG
DOI: 10.1365/s10337-005-0651-3
Keywords
column liquid chromatography; clenbuterol in plasma; doping in sports; anabolic agents
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The quantitative determination of clenbuterol in human plasma and urine is of particular interest far various fields such as clinical and forensic research and doping controls. A simple and rapid sample preparation procedure based on liquid-liquid extraction with subsequent re-extraction followed by liquid chromatography and electrospray ionization tandem mass spectrometry allowed the determination of clenbuterol in urine and plasma at detection and quantification limits of 0.1 ng mL(-1) and 0.2 ng mL(-1), respectively, with recoveries ranging from 85-96%. The fast and robust nature of the assay provides a rapid and cost-effective alternative to established procedures utilizing solid-phase extraction strategies.
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