Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 192, Issue 7, Pages 1237-1244Publisher
OXFORD UNIV PRESS INC
DOI: 10.1086/444544
Keywords
-
Categories
Funding
- NIAID NIH HHS [AI43492] Funding Source: Medline
Ask authors/readers for more resources
Background. We examined the importance of the adaptive and innate immune responses in the resolution of an acute bacterial sinus infection in mice. Methods. Recombinase-activating gene knockout (RAG-1(-/-)) ( no lymphocytes) and C57BL/6 (wild-type) mice were infected with Streptococcus pneumoniae. For determination of the cell type involved, lymphocytes from mice were adoptively transferred into RAG-1(-/-), C57BL/6 (all lymphocytes), B cell-deficient, and T cell-deficient mice. The degree of infection and inflammation was determined by quantification of S. pneumoniae from nasal lavage and analysis of sinus tissue, respectively. Results. In C57BL/6 mice, both the infection and inflammation resolved in 21 days, whereas neither resolved in RAG-1(-/-) mice. When C57BL/6 lymphocytes were adoptively transferred into RAG-1(-/-) mice, resolution of the infection and inflammation occurred. Mice without B cells were able to clear the infection, whereas mice without T cells could not clear it. In vitro stimulation of the draining lymph nodes of the infected mice by use of heat-killed S. pneumoniae led to the production of interferon (IFN)-gamma. Flow-cytometric analysis of lymphocytes obtained from sinus mucosa and draining lymph nodes showed an increase in the number of type 1 T helper cell-like cells over that in control mice. Conclusions. RAG-1(-/-) mice with innate immunity but no lymphocytes contain - but cannot clear - a bacterial sinus infection. Lymphocytes transferred to RAG-1(-/-) mice clear the infection. The sinus mucosa and draining lymph nodes show an increase in T cells generating IFN-gamma. These data demonstrate that T cells are essential in clearing an acute S. pneumoniae bacterial sinus infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available