Journal
CYTOMETRY PART A
Volume 67A, Issue 2, Pages 144-150Publisher
WILEY
DOI: 10.1002/cyto.a.20159
Keywords
telomeres; three-dimensional imaging; image processing; fluorescence microscopy; fluorescence in situ hybridization
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Background: Quantitative analysis can be used in combination with fluorescence microscopy. Although the human eye is able to obtain good qualitative results, when analyzing the spatial organization of telomeres in interphase nuclei, there is a need for quantitative results based on image analysis. Methods: We developed a tool for analyzing three-dimensional images of telomeres stained by fluorescence in situ hybridization in interphase nuclei with DNA counterstained with 4',6-diamidino-2-phenytindole. After deconvolution of the image, we segmented individual telomeres. From the location of the telomeres we derived a distribution parameter p(T),, which indicated whether the telomeres were in a disk (PT >> 1) or not (p(T) approximate to 1). We sorted mouse lymphocyte nuclei and measured P-T. We also performed a bromodeoxyuridine synchronous cell sorting experiment on live cells and measured P-T at several instances. Results: Measuring PT for nuclei in G0/G1, S, and G2 produced 1.4 +/- 0.1, 1.5 +/- 0.2, and 14 +/- 2, respectively, showing a significant difference between G2 and G0/G1 or S. For the bromodeoxyuridine synchronous cell sorting experiment, we found a cell cycle dependency Of PT and a correlation between PT and an observer. Conclusions: In this study we present a quantitative method to characterize the organization of telomeres using three-dimensional imaging, image processing, and image analysis. (c) 2005 international Society for Analytical Cytology.
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