Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 58, Issue 5, Pages 2952-2957Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.02418-13
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Funding
- Ministry of Health of the Czech Republic [NT11032-6/2010]
- Charles University Research Fund [P36]
- The Education for Competitiveness Operational Programme (ECOP) [CZ.1.07/2.3.00/30.0022]
- ESF
- government budget of the Czech Republic
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This study exploited the possibility to detect Citrobacter freundii-derived CMY-2-like cephalosporinases in Enterobacteriaceae clinical isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Periplasmic proteins were prepared using a modified sucrose method and analyzed by MALDI-TOF MS. A ca. 39,850-m/z peak, confirmed to represent a C. freundii-like beta-lactamase by in-gel tryptic digestion followed by MALDI-TOF/TOF MS, was observed only in CMY-producing isolates. We have also shown the potential of the assay to detect ACC- and DHA-like AmpC-type beta-lactamases.
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