Journal
GENES & DEVELOPMENT
Volume 19, Issue 19, Pages 2378-2387Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.344505
Keywords
transcription initiation; RNAP structure; rRNA promoters; stringent response; omega
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Funding
- NIGMS NIH HHS [R37 GM041376, R37 GM037048, R01 GM037048, GM41376, R01 GM041376, GM37048] Funding Source: Medline
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Previous studies have come to conflicting conclusions about the requirement for the to subunit of RNA polymerase in bacterial transcription regulation. We demonstrate here that purified RNAP lacking omega does not respond in vitro to the effector of the stringent response, ppGpp. DksA, a transcription factor that works in concert with ppGpp to regulate rRNA expression. in vivo and in vitro, fully rescues the ppGpp-unresponsiveness of RNAP lacking omega, likely explaining why strains lacking w display a stringent response in vivo. These results demonstrate that omega plays a role in RNAP function (in addition to its previously reported role in RNAP assembly) and highlight the importance of inclusion of omega in RNAP purification protocols. Furthermore, these results suggest that either one or both of two short segments in the beta' subunit that physically link omega to the ppGpp-binding region of the enzyme may play crucial roles in ppGpp and DksA function.
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