Journal
POSTHARVEST BIOLOGY AND TECHNOLOGY
Volume 38, Issue 1, Pages 91-98Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.postharvbio.2005.05.011
Keywords
Castanea henryi chestnuts; phenolics; polyphenol oxidase activity; browning reaction; storage treatments
Categories
Ask authors/readers for more resources
Chestnuts of Castanea henryi (Skan) Rehd. et Wils were stored at 4 and -20 degrees C for a duration of 6 months. The effects of such storage treatments on the polyphenol oxidase (PPO) activity and total free phenolics content were investigated. Total phenolics content showed uneven distribution in C. henryi chestnuts. The chestnut PPO was isolated and characterized in terms of optimum temperature (40 degrees C), optimum pH (5.0), substrate specificity (catechol most efficient) and relative molecular weight (69.0 kDa). The PPO specific activity decreased from ca. 1180 to 340 U mg(-1) for chestnuts stored at 4 degrees C and to ca. 300 U mg(-1) for those stored at -20 degrees C. The PPO activity and the storage temperatures in this investigation showed a significant (P < 0.05) correlation. Moreover, the total phenolics content in both embryonic bud and endosperm decreased more during storage at -20 degrees C than at 4 degrees C. We therefore found a statistically significant correlation (P < 0.05) between the phenolics content and the PPO activity in chestnuts stored at these temperatures. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available