Journal
SURFACE & COATINGS TECHNOLOGY
Volume 200, Issue 1-4, Pages 993-995Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.surfcoat.2005.02.001
Keywords
plasma polymer; allylamine; DNA immobilization; hybridization
Ask authors/readers for more resources
DNA attachment on amine functionalized surfaces has been found to depend on the macromolecular architecture of the plasma films, on the amine group density as well as on the immobilization conditions. Surface Plasmon Resonance spectroscopy (SPR) and Surface Plasmon Enhanced Fluorescence Spectroscopy (SPFS) were used to monitor (a) the immobilization of probe oligomers (25 mers) to plasma polymerised allylamine films (ppA) and (b) their subsequent hybridization reactions with fluorophore-labeled 15 mer target oligonucleotides from solution. To avoid unspecific adsorption of target DNA, any unreacted amino groups were neutralized with succinic anhydride according to standard hybridization procedures. SPF spectroscopic investigations allowed for a precise differentiation between hybridization reactions with mismatch 2 (MM2), mismatch I (MM1) and fully complementary (MM0) oligonucleotide target sequences. Complementary target DNA hybridization was shown to have a significantly higher affinity constant (K-A) than MM1 and MM2. Low energy plasma deposited allylamine films appear to act as a 3D matrix showing a high capacity towards DNA immobilization and hybridization reactions. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available