Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 58, Issue 3, Pages 1785-1788Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.02007-13
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Funding
- National Basic Research Program of China [2013CB127200]
- German Federal Ministry of Education and Research (BMBF) through the German Aerospace Center (DLR) [01KI1014D]
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The novel lincosamide resistance gene lnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5 segment, was identified in Streptococcus suis. The gene lnu(E) encodes a 173-amino-acid protein with <= 69.4% identity to other lincosamide nucleotidyltransferases. The lnu(E) gene and its promoter region were de novo synthesized, and Staphylococcus aureus RN4220 carrying a shuttle vector with the cloned lnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.
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