Journal
FEBS LETTERS
Volume 579, Issue 24, Pages 5338-5342Publisher
WILEY
DOI: 10.1016/j.febslet.2005.09.005
Keywords
endothelial progenitor cells; osteoblasts; tissue engineering; sprouting; angiogenesis; spheroid culture
Funding
- NCI NIH HHS [CA45548, CA37392] Funding Source: Medline
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Survival of tissue transplants generated in vitro is strongly limited by the slow process of graft vascularization in vivo. A method to enhance graft vascularization is to establish a primitive vascular plexus within the graft prior to transplanta- tion. Endothelial cells (EC) cultured as multicellular spheroids within a collagen matrix form sprouts resembling angiogenesis in vitro. However, osteoblasts integrated into the graft suppress EC sprouting. This inhibition depends on direct cell-cell-interactions and is characteristic of mature ECs isolated from preexisting vessels. In contrast, sprouting of human blood endothelial progenitor cells is not inhibited by osteoblasts, making these cells suitable for tissue engineering of pre-vascularized bone grafts. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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