Journal
FEBS LETTERS
Volume 579, Issue 24, Pages 5313-5317Publisher
WILEY
DOI: 10.1016/j.febslet.2005.08.055
Keywords
sphingosine-1-phosphate; sphingosine kinase; epidermal growth factor; MCF-7; cell migration; apoptosis
Funding
- NCI NIH HHS [R01CA61774, P30 CA16059] Funding Source: Medline
Ask authors/readers for more resources
Sphingosine-1 -phosphate (S1P) is a potent lysolipid involved in a variety of biological responses important for cancer progression. Therefore, we investigated the role of sphingosine kinase type 1 (SphK1), the enzyme that makes SIP, in the motility, growth, and chemoresistance of MCF-7 breast cancer cells. Epidermal growth factor (EGF), an important growth factor for breast cancer progression, activated and translocated SphK1 to plasma membrane. SphK1 was required for EGF-directed motility. Downregulation of SphK1 in MCF-7 cells reduced EGF- and serum-stimulated growth and enhanced sensitivity to doxorubicin, a potent chemotherapeutic agent. These results suggest that SphK1 may be critical for growth, metastasis and chemoresistance of human breast cancers. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available