Preparation of Ag-Au nanoparticle and its application to glucose biosensor

In this paper, Ag-Au nanoparticles are produced in sodium-bis(2-ethylhexyl)-sulfosuccinate (AOT)-cyclohexane reverse micelle system. The properties of the obtained nanoparticles are characterized with transmission electron microscope (TEM) and UV-vis absorption spectrophotometer. Glucose biosensors have been formed with glucose oxidase (GOx) immobilized in Ag-Au sol. GOx are simply mixed with Ag-Au nanoparticles and crosslinked with a polyvinyl butyral (PVB) medium by glutaraldehyde. Then a platinum electrode is coated with the mixture. The effects of the various molar ratios of Ag-Au particles with respect to the current response and the stability of the GOx electrodes are studied. The experimental results indicate the current response of the enzyme electrode containing Ag-Au sol increase from 0.32 to 19 mu A cm(-2) in the solution of 10 mM beta-D-glucose. In our study, the stability of enzyme electrodes is also enhanced. (C) 2005 Elsevier B.V All rights reserved.