Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 56, Issue 11, Pages 6037-6040Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.01320-12
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Funding
- MRC Programme grant [GO501415]
- MRC [G0801977] Funding Source: UKRI
- Medical Research Council [G0801977] Funding Source: researchfish
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RamA is a transcription factor involved in regulating multidrug resistance in Salmonella enterica serovar Typhimurium SL1344. Green fluorescent protein (GFP) reporter fusions were exploited to investigate the regulation of RamA expression by RamR. We show that RamR represses the ramA promoter by binding to a palindromic sequence and describe a superrepressor RamR mutant that binds to the ramA promoter sequence more efficiently, thus exhibiting a ramA inactivated phenotype.
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