Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 56, Issue 4, Pages 1774-1783Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.05395-11
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Funding
- Russian Foundation for Basic Research [10-04-01345-a, 11-04-12060, 11-04-01314-a, 11-04-01018-a]
- Russian Ministry of Science [16.512.11.2108]
- Federal Agency for Science and Innovations [02.740.11.0706]
- Moscow University [PNR 5.13]
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A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.
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