4.7 Article

Complete Sequence of pJIE143, a pir-Type Plasmid Carrying ISEcp1-blaCTX-M-15 from an Escherichia coli ST131 Isolate

Journal

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 55, Issue 12, Pages 5933-5935

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00639-11

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Funding

  1. Australian National Health and Medical Research Council [512396]
  2. Australian Postgraduate Award
  3. Australian Government Department of Education, Science and Training
  4. New South Wales Office of Science and Medical Research

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pJIE143 (34 kb), from an Escherichia coli ST131 isolate, carries bla(CTX-M-15) but could not be typed using the standard PCR-based replicon-typing primer set. Complete sequencing revealed a backbone with similarity to IncX plasmids, including a pir-like gene encoding a pi-like replication protein and iterons related to those of other IncX plasmids. The 2.971-kb ISEcp1-bla(CTX-M-15)-orf477 Delta transposition unit often found within Tn2 is inserted just beyond the end of pir, flanked by 5-bp direct repeats.

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